Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Diatrizoic Acid (Amidotrizoic acid) 是一种有机碘化不透射线 X 射线造影剂,用于放射诊断,具有对气道进行放射照相成像的潜力。它通过钙失调诱导线粒体更新和氧化应激,激活细胞凋亡。
产品描述 | Diatrizoic Acid (Amidotrizoic acid) is an organic, iodinated radiopaque X-ray contrast medium used in diagnostic radiography. |
激酶实验 | Radiochemical enzyme assays for COX-1 and COX-2: 10 μL of purified COX-1 (0.7-0.8 μg) or COX-2 (3.0 units, 0.3μg) is activated with 50 μL of cofactor solution [l-epinephrine (1.3 mg/mL), reduced glutathione (0.3 mg/mL), and hematin (1.3 mg/mL) in oxygen-free Tris-HCl buffer (pH 8.0)]. The enzyme solution (60 μL) is added to Ibuprofen solutions or DMSO (20 μL) after [14C]arachidonic acid is added in 0.2 mL eight-strip test tubes and preincubated 10 minutes on ice. Samples are incubated for 15 minutes at 37 °C, after which the reaction is terminated by addition of 10 μL of 2 M HCl and 5 μL of carrier solution (PGE2 and PGF2α, 0.2 μg/mL of each in EtOH). The unmetabolized arachidonic acid is separated from the prostaglandin products by column chromatography and eluted with n-hexane-dioxane-glacial acetic acid (70:30:1). The prostaglandin products are then eluted with EtOAc-MeOH (85:15), and the samples are counted in a Packard scintillation spectrometer. IC50 values are obtained by linear regression analysis. |
别名 | Diatrizoate, 泛影酸, Amidotrizoic acid |
分子量 | 613.91 |
分子式 | C11H9I3N2O4 |
CAS No. | 117-96-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (89.59 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.6289 mL | 8.1445 mL | 16.289 mL | 40.7226 mL |
5 mM | 0.3258 mL | 1.6289 mL | 3.2578 mL | 8.1445 mL | |
10 mM | 0.1629 mL | 0.8145 mL | 1.6289 mL | 4.0723 mL | |
20 mM | 0.0814 mL | 0.4072 mL | 0.8145 mL | 2.0361 mL | |
50 mM | 0.0326 mL | 0.1629 mL | 0.3258 mL | 0.8145 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Diatrizoic Acid 117-96-4 Apoptosis Others Inhibitor inhibit Diatrizoate 泛影酸 Amidotrizoic acid inhibitor